3FD9

Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-16
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	144.755, 69.347, 86.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 2.600
R-factor	0.215
Rwork	0.213
R-free	0.26300
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.832
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RESOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.401	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.110	0.390
Number of reflections	23616
<I/σ(I)>		1.7
Completeness [%]	92.5	57.4
Redundancy	8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8	277	0.1 M Bis-Tris (pH 6.8), 0.2 M Ammonium Acetate, and 30% polyethylene glycol 3350 was combined at a 1:1 ratio with 20ExsD solution at 15 mg/ml in 25 mM Tris-HCl (pH 7.4), 0.15 M NaCl, 2 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 277K