3FD4
Crystal Structure of Epstein-Barr virus gp42 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.792, 67.469, 79.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.730 - 2.400 |
| R-factor | 0.21666 |
| Rwork | 0.215 |
| R-free | 0.24226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kg0 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.708 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 14139 | |
| Completeness [%] | 99.4 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.5 | 298 | 30% PEG-3000, 100 mM CHES pH 9.5, 10 mM YCl3, 25 mM tris pH 7.5, 150 mM NaCl, temperature 298K, VAPOR DIFFUSION |
