3FBN
Structure of the Mediator submodule Med7N/31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-01-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9177 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 174.620, 174.620, 117.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.655 - 3.007 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fbi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 0.574 |
| Number of reflections | 13576 | |
| <I/σ(I)> | 32.7 | 5.15 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 11.2 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50mM MES pH 5.6, 1.8M Li2SO4, 10mM MgCl2, vapor diffusion, hanging drop, temperature 293K |
