3FBM
D431N Mutant VP2 Protein of Infectious Bursal Disease Virus; Derived T=1 Particles
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 3 |
Unit cell lengths | 326.725, 326.725, 326.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.294 |
Rwork | 0.294 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gsy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC ((Rigid Body)) |
Refinement software | REFMAC ((Rigid Body)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 206393 | |
<I/σ(I)> | 9.3 | 1.9 |
Completeness [%] | 99.0 | 95.8 |
Redundancy | 5.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 14-17% PEG 4000, 0.1M TRIS pH 9.0, 5% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |