3FBL

Crystal structure of ORF132 of the archaeal virus Acidianus Filamentous Virus 1 (AFV1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-01-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	28.353, 47.576, 60.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 1.950
R-factor	0.16203
Rwork	0.157
R-free	0.19619
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.066
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.600	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.037	0.066
Number of reflections	6349
<I/σ(I)>	136.6	89
Completeness [%]	100.0	100
Redundancy	76	76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12% PEG 8000, 0.025 mM Na2HPO4/KH2PO4, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K