3FBL
Crystal structure of ORF132 of the archaeal virus Acidianus Filamentous Virus 1 (AFV1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.353, 47.576, 60.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.950 |
| R-factor | 0.16203 |
| Rwork | 0.157 |
| R-free | 0.19619 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.066 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.600 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.037 | 0.066 |
| Number of reflections | 6349 | |
| <I/σ(I)> | 136.6 | 89 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 76 | 76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 12% PEG 8000, 0.025 mM Na2HPO4/KH2PO4, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
