3FB9
The crystal structure of the protein with unknown function from Streptococcus pneumoniae TIGR4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.609, 53.118, 72.036 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.760 - 1.800 |
R-factor | 0.19297 |
Rwork | 0.191 |
R-free | 0.23436 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.562 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.760 | 1.846 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.085 | 0.238 |
Number of reflections | 16247 | |
<I/σ(I)> | 29.64 | 8.14 |
Completeness [%] | 99.6 | 97.2 |
Redundancy | 9.4 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M NaCl, 0.1M Tris, 25%PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |