3FAY
Crystal structure of the GAP-related domain of IQGAP1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97924, 0.97900, 0.92523 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 170.133, 42.115, 59.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
Rwork | 0.228 |
R-free | 0.25820 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 20692 | |
<I/σ(I)> | 8.9 | 2.1 |
Completeness [%] | 86.1 | 38.2 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | 20% PEG 2000 methyl ether, 500mM MgCl2, 100mM Tris HCL, pH 8.5, VAPOR DIFFUSION, temperature 277K |