3FAR
Cation-dependent self-cleavage activity in the duplex form of the subtype-B HIV-1 RNA Dimerization Initiation Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-16 |
Detector | MAC Science DIP-2000 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.689, 47.541, 57.735 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.990 - 2.400 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oiy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 8591 | |
<I/σ(I)> | 14.1 | 2.2 |
Completeness [%] | 94.2 | 94.6 |
Redundancy | 2.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 310 | MgCl2, Na Cacodylate, KCl, spermine, MPD, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MgCl2 | ||
10 | 1 | 2 | MPD | ||
11 | 1 | 2 | H2O | ||
2 | 1 | 1 | Na Cacodylate | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 1 | spermine | ||
5 | 1 | 1 | MPD | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | MgCl2 | ||
8 | 1 | 2 | Na Cacodylate | ||
9 | 1 | 2 | KCl |