3FAP
ATOMIC STRUCTURES OF THE RAPAMYCIN ANALOGS IN COMPLEX WITH BOTH HUMAN FKBP12 AND FRB DOMAIN OF FRAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.400, 51.720, 101.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| Rwork | 0.207 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fap |
| RMSD bond length | 0.032 |
| RMSD bond angle | 4.000 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 1.850 | 1.800 |
| Rmerge | 0.053 | 0.325 |
| Number of reflections | 20139 | |
| <I/σ(I)> | 10.4 | 2.3 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 8 | This particular structure is not described in this paper. * |
