3FAN
Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418, 0.9793, 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.358, 49.162, 42.907 |
| Unit cell angles | 90.00, 110.25, 90.00 |
Refinement procedure
| Resolution | 27.910 - 1.900 |
| R-factor | 0.19633 |
| Rwork | 0.194 |
| R-free | 0.24585 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.572 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 | 0.167 |
| Number of reflections | 17461 | |
| <I/σ(I)> | 22.1 | 4.3 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | 0.1M sodium citrate pH 5.3, 0.7M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
