3FA2
Crystal Structure of the BRCA1 Associated Ring Domain (BARD1) Tandem BRCT Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.979, 67.466, 86.783 |
| Unit cell angles | 90.00, 99.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.22605 |
| Rwork | 0.222 |
| R-free | 0.29772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2NTE CHAIN A |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.283 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.045 | 0.578 |
| Number of reflections | 28268 | |
| <I/σ(I)> | 16.77 | 1.22 |
| Completeness [%] | 82.4 | 30.4 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES 15% w/v PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
