3F8U

Tapasin/ERp57 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	74.101, 72.295, 200.193
Unit cell angles	90.00, 94.61, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.247
Rwork	0.245
R-free	0.28500
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.074	0.207
Number of reflections	63580
<I/σ(I)>	19.4	6.5
Completeness [%]	97.4	91.4
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	reservoir contained 100 mM HEPES (@pH 7.5), 20% PEG 3K, 200 mM CaCl2. protein was concentrated to 10mg/ml in 150 mM NaCl, 20 mM Tris (@ pH 7.4), 5% glycerol. The hanging drop was supplemented to contain 100 mM guanidine HCl; seeding was used to obtain larger crystals., VAPOR DIFFUSION, HANGING DROP, temperature 295K