3F8K
Crystal structure of protein acetyltransferase (PAT) from Sulfolobus solfataricus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-03 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97932, 0.97945, 0.94932 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.701, 46.750, 68.624 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.840 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.22100 |
Structure solution method | MAD |
RMSD bond length | 0.070 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.920 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.068 | 0.423 |
Number of reflections | 23521 | |
<I/σ(I)> | 23.1 | 2.5 |
Completeness [%] | 98.6 | 89.2 |
Redundancy | 5.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 12% PEG 20000, 0.05M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |