3F8I
Mouse UHRF1 SRA domain bound with hemi-methylated CpG, crystal structure in space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-20 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.084, 62.416, 113.447 |
Unit cell angles | 90.00, 98.92, 90.00 |
Refinement procedure
Resolution | 34.530 - 2.290 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zo0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.530 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.102 | 0.352 |
Number of reflections | 30041 | |
<I/σ(I)> | 10.5 | 2.3 |
Completeness [%] | 97.5 | 90.5 |
Redundancy | 3.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 20% PEG 3350, 0.4M NaCl, pH 7.0, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 2 | PEG 3350 | ||
4 | 1 | 2 | NaCl |