3F7Q
First pair of Fibronectin type III domains and part of the connecting segment of the integrin beta4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.110, 49.470, 74.360 |
| Unit cell angles | 107.22, 96.06, 106.60 |
Refinement procedure
| Resolution | 22.272 - 1.750 |
| R-factor | 0.1566 |
| Rwork | 0.155 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qg3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.878 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.480 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 50835 | |
| <I/σ(I)> | 16.3 | 3.8 |
| Completeness [%] | 91.6 | 84.8 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1M Tris-HCl, 0.3M NaCl, 24% PEG 200, pH 7.5, VAPOR DIFFUSION, temperature 298K |
