3F6Z
Crystal structure of Pseudomonas aeruginosa MliC in complex with hen egg white lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-10 |
Detector | ADSC |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 175.970, 90.960, 49.730 |
Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.25 |
Rwork | 0.238 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.230 | 2.410 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.098 | 0.208 |
Number of reflections | 34458 | |
<I/σ(I)> | 18.6 | 8.5 |
Completeness [%] | 1.0 | 100 |
Redundancy | 6.8 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 1.0M sodium citrate dehydrate, 0.1M HEPES (pH 7.5) , VAPOR DIFFUSION, HANGING DROP, temperature 287K |