3F6T

Crystal structure of aspartate aminotransferase (E.C. 2.6.1.1) (YP_194538.1) from Lactobacillus acidophilus NCFM at 2.15 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-27
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	89.780, 107.750, 119.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.921 - 2.150
R-factor	0.18
Rwork	0.177
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.665
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.921	29.921	2.150
High resolution limit [Å]	2.150	5.060	2.100
Rmerge	0.147	0.055	0.805
Number of reflections	63504
<I/σ(I)>	9.204
Completeness [%]	99.9	98.8	99.9
Redundancy	3.6	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	277	0.2000M (NH4)2HPO4, 20.0000% PEG-3350, No Buffer pH 7.9, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K