3F6O
Crystal structure of ArsR family transcriptional regulator, RHA00566
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9199 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.543, 65.543, 93.024 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.21692 |
| Rwork | 0.216 |
| R-free | 0.24693 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.393 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.428 |
| Number of reflections | 18153 | |
| <I/σ(I)> | 9.8 | 2.8 |
| Completeness [%] | 97.4 | 93.4 |
| Redundancy | 9.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.1M Bis-tris pH5.5 0.2M MgCl2 25%PEG3350 , VAPOR DIFFUSION, SITTING DROP, temperature 297K |
