3F62
Crystal Structure of Human IL-18 in complex with Ectromelia virus IL-18 Binding Protein
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-03 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.356, 69.210, 104.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.610 - 2.000 |
| R-factor | 0.19089 |
| Rwork | 0.189 |
| R-free | 0.23545 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.377 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.048 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17333 | |
| <I/σ(I)> | 12.66 | 1.5 |
| Completeness [%] | 94.4 | 100 |
| Redundancy | 5.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 30% Peg 3350, 0.5M KCl, 0.1M Sodium Citrate, pH 4.5, pH 7.5, VAPOR DIFFUSION, temperature 298K |
