3F5M
Crystal Structure of ATP-Bound Phosphofructokinase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.896 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.578, 117.570, 176.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.879 - 2.700 |
| R-factor | 0.2268 |
| Rwork | 0.224 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hig |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.259 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.880 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 54525 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 97.9 | 97.8 |
| Redundancy | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 2.3-2.7 M sodium formate, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
