3F4A
Structure of Ygr203w, a yeast protein tyrosine phosphatase of the Rhodanese family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.382, 72.025, 53.514 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 36.010 - 1.800 |
| R-factor | 0.16808 |
| Rwork | 0.166 |
| R-free | 0.21064 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.564 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.295 |
| Number of reflections | 27354 | |
| <I/σ(I)> | 25.682 | 2.6 |
| Completeness [%] | 98.7 | 89.3 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris 8,5, 1.5 M LiSO4 plus 0.015 mg/ml trypsin. Cryoprotected with N-paratone oil, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
