3F41
Structure of the tandemly repeated protein tyrosine phosphatase like phytase from Mitsuokella multacida
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2150,1.2146,1.1579 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.391, 73.304, 161.310 |
Unit cell angles | 90.00, 93.71, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.23 |
Rwork | 0.208 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.462 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | SOLVE (2.12) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.088 | 0.293 |
Total number of observations | 28896 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 96.5 | 91.4 |
Redundancy | 3.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 10% PEG 8000, 2% ethylene glycol, 100 mM Tris, pH 8.5, vapor diffusion, temperature 298 K, VAPOR DIFFUSION |