3F3R
Crystal structure of yeast Thioredoxin1-glutathione mixed disulfide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.530, 38.810, 41.700 |
| Unit cell angles | 72.91, 87.51, 60.58 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.18716 |
| Rwork | 0.183 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fa4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.310 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 0.057 |
| Number of reflections | 17174 | |
| <I/σ(I)> | 31.9 | 22.5 |
| Completeness [%] | 92.7 | 89.3 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 2.2M Ammonium Sulfate, 0.1M Sodium Acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
