3F2O
Crystal Structure of human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1) in complex with a 20-residue VASA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-10 |
Detector | MAR225 |
Wavelength(s) | 1.00001 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.665, 80.654, 86.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.240 - 2.050 |
R-factor | 0.18215 |
Rwork | 0.180 |
R-free | 0.23198 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jk9 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.497 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.240 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.092 | 0.649 |
Number of reflections | 34014 | |
<I/σ(I)> | 10 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG3350, 0.2M NaCl, 0.1M BisTris pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |