3F1Z

Crystal structure of putative nucleic acid-binding lipoprotein (YP_001337197.1) from Klebsiella pneumoniae subsp. pneumoniae MGH 78578 at 2.46 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-04
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	97.420, 105.507, 181.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.881 - 2.460
R-factor	0.194
Rwork	0.192
R-free	0.22800
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.698
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.881	29.881	2.520
High resolution limit [Å]	2.460	11.000	2.460
Rmerge	0.111	0.060	0.696
Total number of observations		4964	37475
Number of reflections	68362
<I/σ(I)>	4.443	9.4	1.1
Completeness [%]	99.8	93	99.7
Redundancy	7.5	6.2	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	31.0% polyethylene glycol 600, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K