3F10
Crystal structure of Clostridium Acetobutylicum 8-oxoguanine DNA glycosylase in complex with 8-oxoguanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 138.500, 138.500, 138.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.790 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.081 | 0.533 |
| Number of reflections | 20809 | 1373 |
| <I/σ(I)> | 3.14 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 18.74 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 285 | 12% PEG-4000, 0.1M NaCitrate, 20%isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
