3EZW
Crystal Structure of a Hyperactive Escherichia coli Glycerol Kinase Mutant Gly230 --> Asp Obtained Using Microfluidic Crystallization Devices
Replaces: 2P3RExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-3 |
| Synchrotron site | SSRL |
| Beamline | BL11-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.116, 114.260, 212.624 |
| Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.1679 |
| Rwork | 0.167 |
| R-free | 0.22478 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glf |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.893 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 250186 | |
| <I/σ(I)> | 36.8 | 6.6 |
| Completeness [%] | 84.0 | 73.2 |
| Redundancy | 5.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microfluidic free interface diffusion | 8.5 | 298 | 20% PEG 1500, 0.3 M MAGNESIUM CHLORIDE, 0.1 M TRIS-HCL, pH 8.5, microfluidic free interface diffusion, temperature 298K |
