3EZI
Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain without Ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-04 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.241, 39.822, 83.947 |
| Unit cell angles | 80.76, 89.57, 85.29 |
Refinement procedure
| Resolution | 19.790 - 1.700 |
| Rwork | 0.189 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.050 | 0.037 | 0.352 |
| Number of reflections | 37137 | ||
| <I/σ(I)> | 13.8 | ||
| Completeness [%] | 92.3 | 98.3 | 78.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | isopropanol, PEG 3350, pH 7.5, vapor diffusion, temperature 277K |
