3EYZ
Cocrystal structure of Bacillus fragment DNA polymerase I with duplex DNA (open form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.663, 94.053, 106.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.211 |
| R-free | 0.24500 |
| Starting model (for MR) | 1l3s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.158 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.084 | 0.041 | 0.408 |
| Number of reflections | 49257 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 93.8 | 98.6 | 67 |
| Redundancy | 3.8 | 4.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | Ammonium Sulfate, MES, MPD, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Sulfate | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | Ammonium Sulfate | ||
| 5 | 1 | 2 | MES | ||
| 6 | 1 | 2 | MPD |
