3EYT
Crystal structure of Thioredoxin-like superfamily protein SPOA0173
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.543, 76.800, 137.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.520 - 1.950 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21000 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.520 | 1.970 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.142 | 0.893 |
| Number of reflections | 51070 | |
| <I/σ(I)> | 20.256 | 1.7 |
| Completeness [%] | 99.9 | 97 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.2M Ammonium sulfate 0.1M Sodium acetate pH 4.6 12% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
