3EYO
Crystal structure of anti-human cytomegalovirus antibody 8F9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-10-20 |
| Detector | SBC-3 |
| Wavelength(s) | 0.96364 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.030, 110.730, 180.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.393 |
| Number of reflections | 44200 | |
| <I/σ(I)> | 13.4 | 2.11 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.6 | 298 | 1.9M Ammonium sulfate, 0.1M CAPS buffer, 5% Dioxane, pH 10.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
