3EY8

Structure from the mobile metagenome of V. Pseudocholerae. VPC_CASS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-08-07
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	38.851, 62.087, 98.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.228 - 1.600
R-factor	0.1579
Rwork	0.156
R-free	0.18730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ey7
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.230	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.045	0.089
Number of reflections	27068
<I/σ(I)>	40.9	17.4
Completeness [%]	84.5	38.9
Redundancy	7	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	294	25% Peg 3350 0.2M LiSo4 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, temperature 294K