3EY7

Structure from the mobile metagenome of V. Cholerae. Integron cassette protein VCH_CASS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	43.029, 67.868, 91.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.754 - 1.600
R-factor	0.1738
Rwork	0.171
R-free	0.21640
Structure solution method	SAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.470	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.084	0.430
Number of reflections	34682
<I/σ(I)>	15.8	3.7
Completeness [%]	96.7	95.3
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	294	9% PEG 8000, 0.2M Ca(OAC), 0.1M NA Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 294K