3EY5
Putative acetyltransferase from GNAT family from Bacteroides thetaiotaomicron.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.798, 50.785, 73.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.300 - 2.150 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.519 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.300 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.113 | 0.549 |
| Number of reflections | 9184 | |
| <I/σ(I)> | 22.3 | 3.17 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 5.4 | 5.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 0.2 M lithium sulfate, 0.1 M CAPS buffer, 1.2 M sodium/potassium phosphate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
