3EXN
Crystal structure of acetyltransferase from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-13 |
Detector | SBC-3 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.197, 52.408, 47.514 |
Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.17825 |
Rwork | 0.176 |
R-free | 0.21899 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.589 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.194 |
Number of reflections | 14476 | |
<I/σ(I)> | 26.3 | 4.8 |
Completeness [%] | 98.6 | 94.9 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 289 | 0.1 M lithium Chloride 25% Peg 6000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |