3EXJ
Crystal Structure of a p53 Core Tetramer Bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 298 |
| Collection date | 2008-04-18 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.740, 68.016, 75.162 |
| Unit cell angles | 90.00, 111.12, 90.00 |
Refinement procedure
| Resolution | 28.700 - 2.000 |
| Rwork | 0.225 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.186 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.465 |
| Number of reflections | 36093 | |
| <I/σ(I)> | 16.5 | 3.6 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 200 mM lithium citrate, 20% PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Lithium Citrate | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 2 | Lithium Citrate | ||
| 4 | 1 | 2 | PEG 3350 |
