3EXH
Crystal structure of the pyruvate dehydrogenase (E1p) component of human pyruvate dehydrogenase complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 257.064, 115.612, 127.592 |
Unit cell angles | 90.00, 113.64, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.444 |
R-factor | 0.167 |
Rwork | 0.165 |
R-free | 0.21100 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.842 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.444 | 6.650 | 2.444 |
Rmerge | 0.155 | 0.050 | 0.693 |
Number of reflections | 123888 | ||
<I/σ(I)> | 14.158 | ||
Completeness [%] | 98.1 | 99 | 96.6 |
Redundancy | 6.7 | 6.2 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% PEG 8000, 140mM NaOAC, 50mM BisTris pH6.5, 10% glucose, 50mM Taurine, 8mM MgCl2, and 10mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |