3EWT

Crystal Structure of calmodulin complexed with a peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2006-09-22
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	40.062, 40.062, 174.431
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.900 - 2.400
R-factor	0.21859
Rwork	0.217
R-free	0.25916
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l7z
RMSD bond length	0.011
RMSD bond angle	1.384
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.042
Number of reflections	6946
Completeness [%]	99.6	97.3
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	293	25%(w/v) PEG8000, 0.2M Sodium Acetate, 0.1M Sodium cacodylate, pH 5.5, EVAPORATION, temperature 293K