3EWS
Human DEAD-box RNA-helicase DDX19 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-21 |
| Detector | Rayonics MX-225 |
| Wavelength(s) | 0.918410 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.580, 47.610, 124.690 |
| Unit cell angles | 90.00, 95.40, 90.00 |
Refinement procedure
| Resolution | 19.859 - 2.700 |
| R-factor | 0.1918 |
| Rwork | 0.188 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.067 | 0.396 |
| Number of reflections | 26518 | |
| <I/σ(I)> | 14.51 | 4.27 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 4.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1M ammonium acetate, 0.1M Bis-Tris, 17% PEG-10000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
