3EWK
Structure of the redox sensor domain of Methylococcus capsulatus (Bath) MmoS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 146.710, 146.710, 43.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.800 - 2.340 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.28300 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.969 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 3.030 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.073 | 0.230 |
| Number of reflections | 17002 | |
| <I/σ(I)> | 33.314 | |
| Completeness [%] | 95.2 | 81.6 |
| Redundancy | 12.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium phosphate and glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium phosphate and glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
