3EW0

The novel 2Fe-2S outer mitochondrial protein mitoNEET displays conformational flexibility in its N-terminal cytoplasmic tethering domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1158
Spacegroup name	P 21 21 21
Unit cell lengths	50.737, 48.476, 59.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.550 - 1.400
R-factor	0.20047
Rwork	0.198
R-free	0.24064
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qh7
RMSD bond length	0.011
RMSD bond angle	1.577
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0063)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.550	1.480
High resolution limit [Å]	1.400	1.400
Number of reflections	26874
<I/σ(I)>	19	4.5
Completeness [%]	92.4	81.4
Redundancy	6.3	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	300	Crystallization Droplets: 100 mM Tris HCl (pH 8.0), 100 mM NaCl, and 16-18% PEG 3000. Reservoir conditions: 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 30 32% PEG 3000 in the reservoir., VAPOR DIFFUSION