3EVZ
Crystal structure of Methyltransferase from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-10-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 46.877, 46.877, 331.412 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.220 - 2.200 |
| R-factor | 0.20195 |
| Rwork | 0.199 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.220 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.111 | 0.331 |
| Number of reflections | 10605 | |
| <I/σ(I)> | 29.1 | 8.6 |
| Completeness [%] | 92.3 | 85 |
| Redundancy | 28.4 | 27.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | D9. 0.1 M Tris pH 8.5, 25% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
