3EVC
Crystal structure of GTP complex of yellow fever virus methyltransferase and S-adenosyl-L-homocysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-09 |
Detector | NOIR-1 |
Wavelength(s) | 1.127 |
Spacegroup name | P 65 |
Unit cell lengths | 104.468, 104.468, 51.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l9k |
RMSD bond length | 0.004 |
RMSD bond angle | 1.200 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 80297 | |
<I/σ(I)> | 14 | 4.3 |
Completeness [%] | 98.9 | 97.5 |
Redundancy | 11.2 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |