3EUU
Crystal structure of the FGFR2 D2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.100, 42.460, 79.620 |
| Unit cell angles | 90.00, 124.99, 90.00 |
Refinement procedure
| Resolution | 22.180 - 2.340 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.22772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3caf |
| RMSD bond length | 0.038 |
| RMSD bond angle | 2.275 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.180 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.109 | 0.425 |
| Number of reflections | 12416 | |
| <I/σ(I)> | 4.9 | 2.2 |
| Redundancy | 3.1 | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.8M ammonium citrate dibasic, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
