3EUP
The crystal structure of the transcriptional regulator, TetR family from Cytophaga hutchinsonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.595, 53.400, 178.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.090 - 1.990 |
| R-factor | 0.18463 |
| Rwork | 0.182 |
| R-free | 0.22937 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.631 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.090 | 2.046 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.110 | 0.670 |
| Number of reflections | 28745 | |
| <I/σ(I)> | 31.62 | 2.76 |
| Completeness [%] | 99.1 | 94.47 |
| Redundancy | 9.2 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG4000, 0.1M MES, 0.2M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
