3EUK
Crystal structure of MukE-MukF(residues 292-443)-MukB(head domain)-ATPgammaS complex, asymmetric dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 172.414, 172.414, 491.587 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.000 |
| Rwork | 0.271 |
| R-free | 0.32400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 8.560 | 4.000 |
| Rmerge | 0.077 | 0.039 | 0.222 |
| Number of reflections | 34959 | ||
| <I/σ(I)> | 11.576 | ||
| Completeness [%] | 92.8 | 98.8 | 71.3 |
| Redundancy | 6 | 8.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8 | 296 | PEG 10000, MgCl2, pH 8.0, hanging drop, temperature 296K |
