3EUC

Crystal structure of histidinol-phosphate aminotransferase (YP_297314.1) from RALSTONIA EUTROPHA JMP134 at 2.05 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	76.579, 93.433, 111.341
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.988 - 2.050
R-factor	0.196
Rwork	0.194
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.671
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.988	29.990	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.102	0.036	0.758
Total number of observations		1966	13492
Number of reflections	50845
<I/σ(I)>	8.8	27.4	1.6
Completeness [%]	100.0	96.7	100
Redundancy	3.6	3.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	0.8M (NH4)2SO4, 0.1M Citrate pH 4.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K