3EUB

Crystal Structure of Desulfo-Xanthine Oxidase with Xanthine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	73.298, 133.176, 142.633
Unit cell angles	96.88, 93.11, 90.02

Refinement procedure

Resolution	33.080 - 2.600
R-factor	0.217
Rwork	0.214
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fiq
RMSD bond length	0.015
RMSD bond angle	1.659
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.420	2.668
High resolution limit [Å]	2.600	2.600
Number of reflections	119504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	batch	7.2	298	PEG 8000, pH 7.2, batch, temperature 298K