3EU4
Crystal Structure of BdbD from Bacillus subtilis (oxidised)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.863, 43.113, 54.718 |
Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
Resolution | 52.200 - 2.300 |
R-factor | 0.19601 |
Rwork | 0.193 |
R-free | 0.24821 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eu3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.262 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.200 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 7198 | |
<I/σ(I)> | 15.7 | 7.3 |
Completeness [%] | 94.8 | 81.2 |
Redundancy | 2.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 25% PEG2000, 0.1M ammonium acetate, 0.1M MES, pH6.5, cryo-protected with 20% Ethylene glycol |