3EU1
Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.678, 66.896, 157.593 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.080 - 3.000 |
| R-factor | 0.21906 |
| Rwork | 0.213 |
| R-free | 0.27796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d1a |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.095 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 9607 | |
| <I/σ(I)> | 2.9 | 0.7 |
| Completeness [%] | 89.7 | 93 |
| Redundancy | 4.12 | 3.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2micro litres of Hb with 2micro litre of well solution containing 25mM of Phosphate buffer at pH 7.5 with 0.5M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
